Plasmid

Part:BBa_K3482024:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


pLPT145 sponge plasmid(Potvin-Trottier, 2016)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 75
    Illegal BglII site found at 2304
    Illegal XhoI site found at 369
    Illegal XhoI site found at 2485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
    Illegal NgoMIV site found at 1640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1580
    Illegal SapI site found at 1790


Design Notes

Temporary


Source

This plasmid originates from the following publication: doi: 10.1038/nature19841.

References